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KMID : 0881720180330050412
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 5 p.412 ~ p.415
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Quantitative Cell Count of Vibrio vulnificus Cells Based on MPN-PCR Method
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Jang Yu-Mi
Park Seul-Ki
Jeong Hee-Jin
Lee Jang-Won
Yoon Yo-Han
Park Kwon-Sam
Shin Il-Shik
Kim Young-Mog
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Abstract
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The objective of this study was to establish a quantitative count method of Vibrio vulnificus cells. Plate count method is often used to count the number of V. vulnificus cell using thiosulfate citrate bile salts sucrose (TCBS) agar plate. However, this method is unsuitable for counting V. vulnificus cells due to growth inhibition and cell injuries in TCBS medium. In this study, we suggested a most probable number-polymerase chain reaction (MPN-PCR) method using alkaline peptone water medium for quantification of V. vulnificus. This MPN-PCR method showed 2 log higher cell number than TCBS agar plate method. Similar result were also found in the control using, Luria-Bertani agar containing 2% NaCl. Thus, this MPN-PCR method can be used an sensitive method for quantitative count of V. vulnificus cells in fish and shellfish samples.
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KEYWORD
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Injured cell, MPN-PCR, Quantitative cell count, Vibrio vulnificus
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